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Bioremediation: EcoClean and GreenClean Series for Bioremediation

Bioremediation: EcoClean and GreenClean Series for Bioremediation

EcoCycle Corporation

EcoClean & Green Clean:        
Bioremediation Formulae
for Soil and Groundwater

- Decontamination of Soil and Groundwater Using Microbes -

Bioremediation is the use of naturally occurring microorganisms to break down hazardous substances namely, chlorinated solvents, petroleum hydrocarbons, cyanides, pesticides, etc. into non-toxic or less toxic substances, like water and carbon dioxide. EcoCleanTM and Green Clean are bioremediation formulae manufactured by EcoCycle Corporation in Japan. Both are made of food grade raw materials. EcoCleanTM provides essential nutrients and energy to microorganisms, which anaerobically decompose various types of toxic contaminants found in soil and groundwater and Green Clean is for aerobic remediation of contaminants in soil and groundwater.

Major Features and Advantages

tech_db_309_4I. Range of Use

EcoCleanTM and Green Clean can treat contaminants in a variety of site conditions except in the highly contaminated (concentration above 500mg/L) and highly acidic environment. EcoCleanTM is mainly used to decompose chlorinated hydrocarbons and heavy metals include hexavalent chromium while Green Clean is used to decompose benzene, toluene, ethylbenzene, and xylene, petroleum hydrocarbons, poly aromatic hydrocarbons, and cyanide compounds. Other contaminants that can be treated by the Company’s products are pesticides, insecticides, and energetics, etc.

II. In-situ Bioremediation

EcoCleanTM and Green Clean are manufactured for in-situ bioremediation, meaning that contaminants are treated on-site. Here, physical removal (dig and dispose) of the contaminated soil and groundwater is not required. The formulae can be used in the premises of factories, under constructions that are operating. EcoClean and Green Clean are essential nutrients to be dissolved in water and injected into the subsurface of a contaminated facility to feed microorganisms, which use the pollutants as a source of respiration and energy. The contaminants are anaerobically or aerobically broken down there so that physical removal of the contaminated soil and groundwater is not required.

III. Low Energy & Cost

Bioremediation requires a little external energy. The installation and operational cost of EcoCleanTM and Green Clean are about 1/3 of the cost of conventional remediation technologies (physical or chemical treatment technologies). The remediation cost of the formulae is between 5,000 to 15,000 yen per m3. Other technologies such as “dig and dispose” or “hot soil” may cost up to 40,000 to 60,000 yen per m3.

IV. Short Remediation Time

The duration of contaminated site remediation is from 3 to 12 months in general, but it is as short as a few months in most sites of contamination. This duration is shorter than that of other technologies whose remediation may take up to several years or decades.

V. Safety

EcoCleanTM and Green Clean are both made of food grade raw materials, which provide well-balanced essential nutrients such as vitamins, amino acids, and minerals. They are safe to the environment.

EcoCleanTM and Green Clean have been widely used because they can be easily adopted in factories of different conditions to clean the contaminated sites. They are used in over 400 sites in Japan, the U.S., Taiwan and in other Asian countries. EcoCycle has formed a company in China and is planning to expand their business in Thailand, India and many other parts of the world.

Technology Data

Conceivable Applications

Our bioremediation products perform In-situ Bioremediation or Purification of soil and groundwater contaminated with chlorinated hydrocarbons and heavy metals with ‘EcoClean’, and petroleum hydrocarbons and cyanide compounds with ‘Green Clean’.

Competitive advantage

For example, EcoClean for bioremediation of Cholorinated Solvents

Performance: EcoClean is a mixture of well-balanced nutrients and energy source for microbes. By application of EcoClean for site remediation, quick anaerobic conditions are met allowing faster and more effective remediation results.

Installation and operational cost (life cycle cost): Low remediation cost, about 1/3 of the cost of conventional methods (physical or chemical treatment technologies)

Applicability: This bioremediation technology is applicable to some cases, where other treatment technologies are not applicable.

Comparison of Different Leading Remediation Technologies with EcoClean is shown on the second page of “Schematic illustration of the technology”


Short remediation time: The products are designed to clean most of the contaminated sites in the span of a few months.

Cleaning Contamination of high concentration: We have successfully degraded contamination with the order of several hundred milligrams/liter, according to Japanese environmental limits, at many sites with DNAPLs (Dense Non-aqueous phase Liquids).

Low energy requirement: Unlike some technologies such as pump and treat, bioremediation has very low energy requirement therefore environmentally green.

Running facilities: Can be applied to clean up the sites under active facilities, factories, and buildings.

Safety: Our products are safe for site bioremediation application, because they are made of food grade materials, and are degraded completely through contaminants degradation by the use of native microorganisms.

Technical maturity

Our bioremediation products have been successfully applied for cleaning over 170 sites contaminated with chlorinated aliphatic hydrocarbons, chromium (VI), petroleum hydrocarbons, & cyanide in Japan, US, Taiwan & other Asian countries.

Conceivable risk

There are no considerable risks as the bioremediation technology is matured, and low cost technology for purification of soil and groundwater contaminants have been applied around the world.. However, there are some cases where bioremediation technology may not be an option where highly acidic conditions exist, with very high contaminant concentrations, making it difficult to sustain active microbial growth, etc.

Information on patent related to this technology

EcoCycleTM Corporation holds several patents on all the proprietary products and injection methods in Japan and/or abroad

Company Data

Name EcoCycle Corporation
Address Nihonbashi Hamacho Building, 2-28-1 Nihonbashi Hamacho, Chuo-ku, Tokyo, Japan
Capital JPY 74,350,000
Contact person Mr. P. S. Reddy
Telephone: +81-3-6661-1875
Fax: +81-3-6661-1876
Number of employees 40 (3-4 for international operations)
Date of company foundation May 1999
The type of business  Manufacturing of Bioremediation Products and Application Design for Soil and Groundwater Purification

Modality of business transaction

The company’s aim is to contribute to the improvement of the regional economy through partnership with local companies, organizations and consulting companies providing greener (ecologically and economically) technologies for contaminated sites’ bioremediation and waste treatment.

Export of product
The company’s products were exported to Taiwan, USA, India, and other Asian Countries, and in the future it wants to establish businesses in all other countries in the world.


EcoCleanTM is wholesome food for specific soil microorganisms. Whereas EcoClean-ETM is an emulsified formula designed to release hydrogen over extended periods of time and useful for cleaning contaminations of high concentrations in silty or clayey soils. Both products are diluted in water and injected into subsurface to stimulate native microorganisms in the contaminated site that are capable of degrading chlorinated hydrocarbons. The products are made of food grade raw materials and is environmentally safe.

Target contaminants : Tetrachloroethylene, trichloroethylene, trichloroethane, carbon tetrachloride, chloroform, dichloroethylenes, dichloroethane, vinyl chloride, dichloromethane, etc.

tech_db_309_6 EcoClean provides essential nutrients (vitamins, amino acids ,etc).
tech_db_309_6 The consortium of native microbes is stimulated
Complete and faster degradation of contaminants
CAHs are degraded

How to use
As shown in the picture below, the bioremediation nutrients are dissolved in water and injected in to the subsurface to stimulate the native microorganism capable of degrading the toxic pollutants.


Also, EcoCycleTM is manufacture nutrients while providing application design for various soil and groundwater pollutants namely, hexavalent chromium, benzene, toluene, ethylbenzene, xylene, petroleum hydrocarbons, and cyanide compounds.

Comparison of Different Leading Remediation Technologies with EcoClean

Microsoft PowerPoint - Product-presentation 101013 [ŒÝŠ·ƒ‚[ƒh]

Contact Person(s)

Mr. P. S. ReddyE-mail:

*Please mention that you saw UNIDO's website when making the first contact with the company.

Registered Category

  • Environmental Technologies : Pollution Prevention and Control